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KMID : 0895820050150010127
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Journal of Oriental Rehabilitation Medicine
2005 Volume.15 No. 1 p.127 ~ p.141
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Effects of Gamichangbaek-san(Jiaweichangbai-san) on Anti-inflammatory, Analgesic, Anti-febrile Activities and Immune Responses in Carrageenan-induced Arthritic Animals
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Han Young-Gyu
Park Young-Hoi
Keum Dong-Ho
Lee Myeong-Jong
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Abstract
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Objectives: This study was performed to investigate the effects of Gamichangbaek-san(Jiaweichangbai-san) on anti-inflammatory, analgesic, anti-febrile and immune response on the arthritis of carrageenan-induced animals.
Methods and Materials: Rats were classified into control and sample groups which are 7 individuals each for the experiments about anti-inflammatory and anti-febrile. Each of the 7 mice were classified into normal, control, sample groups for the analgesic experiments. Gamichangbaek-san(Jiaweichangbai-san) was administered to sample group and normal saline was administered to normal and control groups. Arthritis was induced by injection of 1% carrageenan 0.1㎖ and Gamichangbaek-san(Jiaweichangbai-san) was administered after 30 minutes. The change of edema in Carrageenan-induced Arthritic Rats’ Paws was measured after 1 hour and 5 hours from the injection of carrageenan with Plethysmometer(7150, UGO BASILE, Italy) by Winter’ method. WBC, Lymphocyte and ESR were measured by heart puncture and CD4+ T cell, CD8+ T cell and CD4+/CD8+ T cell ratio were measured from the spleen tissue. Writhing syndrom was measured with Tail flick unit(UGO BASILE, Italy) in the experiments conducted to check the analgesic activity. The temperature of the paws of carrageenan-induced arthritic rats was measured by Laser thermometer. Rectal temperature was measured by Yeast’s method in anti-febrile experiments. Immune response was measured by CD4+, CD8+ T cell ratio and CD4+/CD8+ T cell ratio.
Results:
1. It was recognized that Gamichangbaek-san(Jiaweichangbai-san) decreased the increase rate of Paw Edema effectively with statistical significance.
2. It was recognized that Gamichangbaek-san(Jiaweichangbai-san) decreased WBC, Lymphocyte and ESR with statistically high significance.
3. It was recognized that Gamichangbaek-san(Jiaweichangbai-san) did not show significant analgesic effect, but the Pressure pain threshold of the paws was increased with statistical significance.
4. It was recognized that Gamichangbaek-san(Jiaweichangbai-san) decreased rectal temperature effectively and had an anti-febrile effect about the febrile of a joint with statistical significance.
5. It was recognized that Gamichangbaek-san(Jiaweichangbai-san) increased CD4+ T cell ratio with statistically high significance and increased CD8+ T cell ratio with statistical non significance but increased CD4+/CD8+ T cell ratio effectively with statistical significance, too.
Conclusions: According to the above results, it can be concluded Gamichangbaek-san(Jiaweichangbai-san) showed the treatment effects on the artificial arthritis resulted from carageenan in rats and mice and it is suggested that more interest and study in the security for the clinical use were needed.
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KEYWORD
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Gamichangbaek-san(Jiaweichangbai-san), Carrageenan, Arthritis, Anti-inflammatory function, Analgesic effect, Anti-febrile function, Immune response
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